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Calahorra M; Sánchez N; Peña A (2018)

INFLUENCE OF PHENOTHIAZINES, PHENAZINES AND PHENOXAZINE ON CATION TRANSPORT IN CANDIDA ALBICANS

J APPL MICROBIOL ():
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© 2018 The Society for Applied Microbiology Aims: (i) To analyse the increase in calcium ion uptake caused by several cationic dyes on Candida albicans, (ii) to postulate a mechanism, (iii) to define the effects of Zn ions on the phenomenon, and (iv) to propose the use of the dyes or their derivatives against C. albicans. Methods and Results: Cells were grown in yeast peptone dextrose medium and starved. We measured the hydrophobic solvent/water partition coefficients and the dyes uptake by the cells and found no correlation with their hydrophobicity. Most of the dyes caused an increase in K+efflux (in correlation with a decrease in86Rb+uptake), and a raise in Ca2+uptake except for those used as Zn salts, but not of their HCl salts. Respiration and acidification of the medium were modified only with few dyes and interestingly, when exposing cultures to nile blue, neutral red and toluidine blue ZnCl2a decrease in C. albicans growth was observed. Conclusions: We propose a general mechanism for the stimulation of Ca2+uptake by the dyes used. Some of the dyes tested might be used as agents against C. albicans, probably combined with other agents. Moreover, the effects of Zn ions on Ca2+uptake and on cell growth open possibilities of further studies, not only of their effects, but also of the mechanism of Ca2+transport in C. albicans and other yeasts. Significance and Impact of the Study: This study, in conjunction with previously published results, contribute to the basic research regarding ion transport in C. albicans and the role of zinc in this process. Besides, suggests the additional use of dyes, along with other antifungals agents, as combined therapy against candidiasis. Derived dyes from those used also might be possible therapeutic agents against this disease.