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Ceballos L; Asanov A; Vaca L (2018)

SINGLE-CHANNEL SINGLE-MOLECULE DETECTION (SC-SMD) SYSTEM

Methods in Molecular Biology 1843():189-201
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© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The combination of single-molecule fluorescence imaging and electrophysiology provides a powerful tool to explore the stoichiometry and functional properties of ionic channels, simultaneously. Here, we describe a typical SC-SMD experiment from the preparation of plasmids containing the genes encoding for the channels of interest fused to fluorescent proteins to the use of the SC-SMD system for simultaneous patch clamping and single-molecule determinations.