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Heras-Romero,Y; Morales-Guadarrama,A; Santana-Martínez,R; Ponce,I; Rincón-Heredia,R; Poot-Hernández,AC; Martínez-Moreno,A; Urrieta,E; Bernal-Vicente,BN; Campero-Romero,AN; Moreno-Castilla,P; Greig,NH; Escobar,ML; Concha,L; Tovar-Y-Romo,LB (2022)

IMPROVED POST-STROKE SPONTANEOUS RECOVERY BY ASTROCYTIC EXTRACELLULAR VESICLES.

Mol Ther 30():798-815
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Spontaneous recovery after a stroke accounts for a significant part of the neurological recovery in patients. However limited, the spontaneous recovery is mechanistically driven by axonal restorative processes for which several molecular cues have been previously described. We report the acceleration of spontaneous recovery in a preclinical model of ischemia/reperfusion in rats via a single intracerebroventricular administration of extracellular vesicles released from primary cortical astrocytes. We used magnetic resonance imaging and confocal and multiphoton microscopy to correlate the structural remodeling of the corpus callosum and striatocortical circuits with neurological performance during 21 days. We also evaluated the functionality of the corpus callosum by repetitive recordings of compound action potentials to show that the recovery facilitated by astrocytic extracellular vesicles was both anatomical and functional. Our data provide compelling evidence that astrocytes can hasten the basal recovery that naturally occurs post-stroke through the release of cellular mediators contained in extracellular vesicles.